Journal: Pediatric Research

Article Title: Ontogeny of MMPs and TIMPs in the Murine Neocortex

doi: 10.1203/pdr.0b013e3181973aee

Figure Lengend Snippet: Figure 2. MMP-2 protein level assayed by ELISA in the neocortex of five mouse strains at embryonic (E) and postnatal (P) ages. (A, BALB/c; B, C3Hen; C, C57BL/6; D, FVB; E, Swiss; and F, summary of all strains (ANOVA 2). Square, Balb; up-triangle: C3Hen; down-triangle: FVB; rhombus: C57BL/6; and circle: Swiss). Six to ten animals were studied in duplicate at each age (males, 3–5; and females, 3–5). Bars represent mean MMP-2 protein concentration SEM. Asterisks indicate statistically significant differences from the P60 group. *p 0.05, **p 0.01 by ANOVA 1 with Dunnett’s (A–E) or Bonferroni’s (F) posttest.

Article Snippet: Quantikine human-mouse MMP-2, human TIMP-2, and mouse TIMP-1 commercial ELISA kits (R&D System, Lille, France) were used as directed.

Techniques: Enzyme-linked Immunosorbent Assay, Protein Concentration

Journal: Pediatric Research

Article Title: Ontogeny of MMPs and TIMPs in the Murine Neocortex

doi: 10.1203/pdr.0b013e3181973aee

Figure Lengend Snippet: Figure 4. TIMP-1 protein level determined by ELISA in the neocortex of Swiss mice at various embryonic (E) and postnatal (P) ages. Six to ten animals (males, 3–5; and females, 3–5) were studied in duplicate at each age. Bars represent mean TIMP-1 protein concentration SEM. Asterisks indi- cate statistically significant age differences from the P60 group. **p 0.01 by ANOVA 1with Dunnett’s posttest.

Article Snippet: Quantikine human-mouse MMP-2, human TIMP-2, and mouse TIMP-1 commercial ELISA kits (R&D System, Lille, France) were used as directed.

Techniques: Enzyme-linked Immunosorbent Assay, Protein Concentration

Journal: Journal of Dental Sciences

Article Title: Age-related changes in salivary biomarkers

doi: 10.1016/j.jds.2013.11.002

Figure Lengend Snippet: Figure 1 Scattergrams representing the correlation between some of the tested salivary features and/or biomarkers: correla- tions between (A) collagenase type-I and salivary flow rate, (B) a-amylase and calcium, (C) a-amylase and TIMP-I, and (D) colla- genase type-I and MMP-8. MMP-8 Z matrix metalloproteinase-8; TIMP-1 Z tissue inhibitor of metalloproteinase.

Article Snippet: The supernatant was collected and stored at 80 C until further analysis of the following salivary biomarkers: Ca (AA-630, an atomic absorption spectrometer; Shimadzu Corporation, Kyoto, Japan), aamylase (EnzyChrom, an a-amylase assay kit; BioAssay Systems, Hayward, CA, USA), GSH and GSSG (GSSG/GSH Quantification Kit; Dojindo Laboratories, Kumamoto, Japan), matrix metalloproteinase-8 (MMP-8; Quantikine Human Total MMP-8 ELISA Kit; R&D Systems Inc., Minneapolis, MN, USA), collagenase type-I (Type-I Collagenase Assay Kit; Primary Cell Co., Ltd., Sapporo, Japan), and tissue inhibitor of metalloproteinase (TIMP-1; Quantikine Human TIMP-1 Immunoassay; R&D Systems Inc.).

Techniques:

Journal: PLoS ONE

Article Title: Serum Biomarkers Associated with Clinical Outcomes Fail to Predict Brain Metastases in Patients with Stage IV Non-Small Cell Lung Cancers

doi: 10.1371/journal.pone.0146063

Figure Lengend Snippet: Distribution of serum biomarkers and other factors by presence of brain metastases at baseline or development of subsequent brain metastases after baseline.

Article Snippet: The quantitative sandwich enzyme immunoassay was used to assess Human TIMP1 (Quantikine® R&D System , Minneapolis , Minnesota) .

Techniques: Biomarker Discovery

Journal: Translational oncology

Article Title: IGFBP3 inhibits tumor growth and invasion of lung cancer cells and is associated with improved survival in lung cancer patients.

doi: 10.1016/j.tranon.2022.101566

Figure Lengend Snippet: Fig. 1. Analysis of IGFBP3 expression in H1299 cells. pIRES2-EGFP-IGFBP3 was transfected into H1299 cells. At 24 h post-transfection, IGFBP3 was analyzed (a) by Western Blot in H1299 lysates and supernatants, and (b) by ELISA in supernatants. (c) The transfection rate was determined by flow cytometry, and (d) the function of secreted IGFBP3 was validated by its ability to bind free IGF-1. Two-sided P-value < 0.05 was considered statistically significant (n = 7; *P < 0.05).

Article Snippet: Concentrations of all factors were measured using specific ELISA assays (Human IGFBP-3 DuoSet ELISA, Human IGF-I/IGF-1 Quantikine ELISA Kit, Human Total MMP-1 DuoSet ELISA, Human Total MMP-3 DuoSet ELISA, Human MMP-9 DuoSet ELISA, and Human TIMP-1 DuoSet ELISA from RD-Systems).

Techniques: Expressing, Transfection, Western Blot, Enzyme-linked Immunosorbent Assay, Flow Cytometry